WebSerum-Free Cell Freezing MediumBAMBANKER is a serum-free medium for long-term freezing at -80 degrees C and preservation of valuable culture cells such as tumor cells … Web7. Phosphate-Buffered Saline (1X PBS) (Cat. No. BSS-1005-B) 8. ENStem-ATM Neural Freezing Medium (1X) (Cat. No. SCM011) 9. Accutase (Cat. No. SCR005) 10. EmbryoMax …
Patent 2787016 Summary - Canadian Patents Database
Webis an animal origin-free (AOF), chemically defined medium that contains no proteins, hydrolysates, or components of unknown composition. CD OptiCHO ™ is formulated … WebMar 2, 2013 · Cat. No. 11782-018 Size: 1 ml Store at -70°C. Do not store in liquid nitrogen. Information for European Customers: These cells are genetically modified and contain plasmid-derived DNA sequences. As a condition of sale, this product must only be used in accordance with all applicable local legislation and guidelines including EC crystal freeze dry
Guaiacol Detection Kit and Va-YSG Medium
WebOnly FGF-2 is needed if using Complete ES Cell Medium (Cat. No. ES-101-B). Sterile filter with 0.22 μm filter. B. ECM Coating of Flasks 1. Thaw Matrigel™ (Fisher Cat. No. CB-40234) on ice at 4°C. Do not thaw Matrigel™ at room temperature as this will lead to unwanted gelling. 2. Dilute Matrigel™ 1:50 in chilled 1X PBS (Cat. No. BSS- WebMake sure to supplement FreeStyle CHO Expression Medium with L-glutamine (Cat. no. 25030-081) to a final concentration of 8 mM. If large numbers of cells are needed, seed cultures at 0.5 106 cells/ml and use cells as soon as they reach a density of 5 106 cells/ml (34 days). ... Place cells on selective medium (CD OptiCHO Medium, Cat no. 12681 ... WebMedium (Cat. No. 22400-105) also supplemented with 10% FBS. Cells were kept in a humidified incubator at 5% CO 2 and 37°C until 90–95% confluency. Cells were then rinsed with Gibco™ Dulbecco’s Phosphate-Buffered Saline (DPBS, Cat. No. 14190-250), followed by dissociation with Gibco ™ TrypLE Express Enzyme (Cat. No. 12605-036). crystal free svg