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Psichecktm-2 vector

Webgastric cDNA and inserted into the psiCHECKTM-2 vector (Promega, Madison, WI, USA). The mutant-type SOX4 3'-UTR was generated using QuikChange Site-Directed Mutagenesis kit (Stratagene, La Jolla, CA, USA) as per the manufac - turer's protocol, and then inserted into the psiCHECKTM-2 vector. For the dual-luciferase reporter assay, SGC-7901 WebYou have 2 remaining attempts. You have 1 remaining attempt. Contact Customer Service. Forgot Password? Close Account Locked. To protect your privacy, your account has been …

MicroRNA124 Regulated Neurite Elongation by Targeting OSBP

WebBy cloning the 3’UTRs of collagen IV subunits into the psiCHECKTM-2 vector, we found that seven of the eight sites in the Col4a3–Col4a6 gene complementary to miR-29 were significantly repressed by miR-29a, b (except for the 7774–7781 of Col4a3 gene). The inhibitory efficiency of miR-29a, b on these seven sites was between 27% and 57%. WebPromega psichecktm 2 vector Psichecktm 2 Vector, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, … clark toys funko pop https://urschel-mosaic.com

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http://www.youbio.cn/product/vt1400 WebHEK293T cells were transfected with psiCHECKTM-2-Wt-IGF2BP2 or psiCHECKTM-2-mut-IGF2BP2, together with miR-138 mimics, inhibitor or the negative control (GenePharma, China) by Lipofectamine 2000 (Invitrogen, USA). Forty- eight hours later, relative luciferase activities were assessed using the Dual-Luciferase Reporter Assay System (Promega, USA). WebAug 1, 2024 · The binding site of miR-509-5p to the TRAF6 3'-UTR region and identification of psiCHECKTM-2 Vector double digests. (A)The binding site of miR-509-5p to the TRAF6 3'-UTR region. Note: The underlined area indicates the target gene mutation site. (B) PCR analysis of recombinant bacteria liquid. clarktoys maintenance

Shuffle T7 Express E.coli Strain大肠杆菌表达菌株及感受态细胞

Category:Promega psiCHECK Vectors psiCHECK -2 …

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Psichecktm-2 vector

miR-509-5p anti-infection response for mycoplasma

WebPsicheck Tm 2 Dual Luciferase Reporter Vector, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, …

Psichecktm-2 vector

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WebMar 20, 2024 · The psiCHECKTM-2 vector with the cloned miR-200c binding site of XIST, named XIST-wild type (WT), was co-transfected with miR-200c mimics or mimics NC using the Lipofectamine 2000 transfection reagent. The mutation-carrying psiCHECKTM-2 vector with a different sequence of the miR-200c binding site, named as the XIST-mutation (Mut), … WebDec 19, 2024 · By cloning the 3’UTRs of collagen IV subunits into the psiCHECKTM-2 vector, we found that seven of the eight sites in the Col4a3–Col4a6 gene complementary to miR-29 were significantly repressed...

WebEnter the email address you signed up with and we'll email you a reset link. WebThe 3 -UTR sequence of PPARγwas cloned and inserted into the PsicheckTM-2 vector to generate PsicheckTM- 2-PPARγ-WT.ThemiR-377bindingsiteinPPAR wasthenmutated,andthemutantfragmentwas 2-PPARγ-MT.Forty-eighthoursaftertheaforemen- tioned constructs were transfected, the luciferase activities …

WebApr 11, 2024 · Vector - CAPL - CAN x 总线信息获取. 在CAN& CANFD 测试中，我们经常需要获取到CAN总线的负载、错误帧、过载帧、发送错误等等CAN总线上面的信息，这些信息如此重要，但是如果真的要写代码去实现也是相当不易的，那我们该如何去获取到的呢？. 下面我们就来一起看下 ... WebMyocyte enhancer factor (MEF2) is a family of transcrip- tion factors that binds to an A/T-rich DNA element in the promoter regions of muscle-specificgenes(12). MEF2 exerts a diversity of function during myogenic differentiation and is involved in the control of various aspects of muscle fiber properties (13).

Web(Chuzhou, Anhui, China) and inserted into the psiCHECKTM-2 vector (Promega, Madison, WI). HEK293T cells were seeded in 24-well plates, and Lipofectamine 2000 was used to …

WebFrom the psiCHECK-2 vector (Promega, Cat. No. C8021), a 231-nucleotide (nt) region was selected from the firefly luciferase gene sequence available for (5) Tumor elimination by … download finding nemo in hindi hdWebpsiCHECK™-2 Vector is designed to provide a quantitative and rapid approach for optimization of RNA interference (RNAi). The vectors enable the monitoring of changes in … download finding nemoWebAug 18, 2024 · The 3′-UTR sequence of PPARγ was cloned and inserted into the PsicheckTM-2 vector to generate PsicheckTM-2-PPARγ-WT. The miR-377 binding site in … clark tracks ltdWebShuffle T7 Express E.coli Strain大肠杆菌表达菌株及感受态细胞. 价 格： ¥0. 货 号： 3544763. 产 地： 北京. 点击询问 我要采购. 竭诚为您服务！. download finding nemo dubbing indonesiaWebMar 20, 2024 · This sequence was linked to the polyclonal site of the psiCHECKTM-2 vector (Promega, Madison, WI, USA) through the double-enzyme digestion of XhoI and NotI, and the wild-type (psiCHECK2-TEAD1 3’UTR WT) and mutant-type (psiCHECK2-TEAD1 3’UTR MT) dual luciferase reporter vector of the TEAD1 gene were constructed by the InFusion method. clark tracey quintet new worldWebpsiCHECKTM-2-vector and 50nM of the miR-876-5p or mimic-NC using ExFect®2000 Transfection Reagent (Vazyme Biotech Co., Ltd). Cell lysates were prepared using Passive Lysis Buffer (Promega) 48h after transfec-tion, and luciferase activity was measured using the Dual-Luciferase Reporter Assay (Promega). Experiments were repeated three times. downloadfind.php actionWebFeb 8, 2024 · PsiCHECKTM‐2 vector without inserted gene was used as negative control (NC) plasmid. The inserted sequences were listed in Table. Table.1. 1. Following the procedures provided by Ribobio and Promega, A7r5 cells were co‐transfected with PsiCHECKTM‐2 vector (WT/MUT/NC) and oligonucleotides (miR‐137 mimic or mimic … download finepix viewer for windows 10