WebbThe Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate is a sensitive, luminolbased enhanced - chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. SuperSignal West Femto Substrate enables femtogram detection of antigen by oxidizing luminol in the presence of HRP and peroxide. WebbThermo Fisher Scientific enables our customers to make the world healthier, cleaner and safer.
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Webb10 juli 2024 · Membranes were blocked in the Starting Block T20 (TBS) blocking buffer (Thermo Scientific, #37543) for 1 h at room temperature, followed by an overnight incubation with the primary antibody (goat anti-mouse EphB4, R&D Systems, #AF446, dilution 1:500) at 4 °C. WebbA34095 Popular Applications & Techniques Shop All Products Services Support Order Status Quick Order Sign in Search All Error: Sorry, this product (A34095) is not web … proform lifestyler treadmill manual
Co-immunoprecipitation and semi-quantitative immunoblotting for …
Webb13 okt. 2024 · Rabbit or mouse secondary antibodies conjugated to horseradish peroxidase (Jackson Immunochemicals) were used at 1:10,000 dilution and treated with Pierce ECL Western Blotting Substrate (Thermo Scientific: 32106), SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific: 34095), or Amersham ECL Prime Western … WebbThermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein level … Webb20 jan. 2024 · Signals were detected using SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific 34095). Data were collected with a UVP Analytik Jena ChemStudio system and analyzed using ImageJ. See Supplementary Data File for a list of all antibodies used in this study and their dilutions. proform light blue joint compound